Specialized Laboratories

The Central Research Laboratory has been exclusively developed to promote and participate in research at the institute. The lab conducts several advanced tests such as Polymerase Chain Reaction (PCR), Western Blot, UV-Visible spectrophotometry,chromatography, electrophoresis and molecular techniques (DNA extraction). Confirmation of Sulphas poisoning and PCR based detection of fungi in corneal scrapings are two new techniques which have been developed by the lab. Future plans include Intra-Uterine Insemination Lab, Cryo-Preservative lab, Stem cell/Tissue culture lab, Genetic test lab and Molecular forensic laboratory.

Highlights of Central Research Lab :
1. Work Diagnosis of Sulphas poisoning by detection of phosphine gas with silver nitrate embeded paper
  Status of the project Accomplished (new routine test at SRMS-IMS)
  Significance Diagnosis/ publication
2. Work Diagnosis of MTB using conventional PCR from clinical samples
  Status of the project Accomplished but not started as routine as the physician relied on Real time PCR.
  Significance Diagnosis
3. Work Molecular diagnosis of fungus (Aspergillus, Candida and Fusarium) in corneal scrapings from patients suspected with fungal keratitis.
  Status of the project Accomplised(new routine test at SRMS-IMS)
  Significance Diagnosis/ publication
4. Work Diagnosis of Wilson’s disease mutation by molecular method
  Status of the project Project in pipeline
  Significance Diagnosis/ publication
5. Work DNA fingerprinting and development of test for other potential poisons.
  Status of the project Project in pipeline
  Significance  
6. Work PCR Test for HPV in cervical and oral cancer tissues
  Status of the project Accomplished (New Routine test at SRMS- IMS )
  Significance Diagnosis/ publication
7. Work PCR test for HSV in tears and corneal scrapping from patients of keratitis
  Status of the project Accomplished (New Routine test at SRMS- IMS )
  Significance Diagnosis/ publication
8. Work PCR test for Malaria parasite in suspected patients
  Status of the project Accomplished (New routine test at SRMS –IMS)
  Significance Diagnosis/ publication
9. Work Electrophoresis of Hemoglobin from anemic patients (Thalassemia, Sickle cell anemia etc )
  Status of the project Accomplished (New routine test at SRMS-IMS)
  Significance Diagnosis/ publication
10. Work Electrophoresis of serum proteins in suspected cases of Multiple Myeloma
  Status of the project Accomplished (New routine test at SRMS IMS )
  Significance Diagnosis/ publication
11. Work Real Time PCR for MTB, HPV-16, HPV-18,HPV-33
  Status of the project Accomplished ( New routine test at SRMS,IMS)
  Significance  
12. Work Real Time PCR Test for Viral load of HBS Ag, HCV, Dengue, Bird Flue, Swine Flue, HLAB-27.
  Status of the project Project in pipeline
  Significance Diagnosis/ publication

1. Work Evaluation of 5 common muations of beta-thalassemia by ARMS-PCR
  Status of the project Project submitted for approval
  Significance Diagnosis/ publication
2. Work Biochemical basis of Obesity
  Status of the project Project submitted for approval
  Significance Publication
3. Work Nutritional therapy for obesity
  Status of the project Project submitted for approval
  Significance Publication
4. Work Induction of pluripotent stem cells and its applications
  Status of the project Project submitted for approval
  Significance Treatment / publication
5. Work Role of serum leptin and serum FFA level in the Development of obesity and obesity caused DM Type-II
  Status of the project Accomplished the project
  Significance  
6. Work Role of serum adiponectin and serum Tumor Neurosis Factor α in obesity and obesity caused DM Type-II
  Status of the project Accomplished
  Significance Publication
7. Work Role of serum PPAR-¥ level in obesity and obesity Caused DM Type-II
  Status of the project Project in pipeline
  Significance  
8. Work Role of serum IgE and serum cationic eosinaphil Protein level inasthma
  Status of the project Accomplished
  Significance Publication/ Treatment
9. Work Level of oxidant in the children exposed to hyper Oxidative stress
  Status of the project Accomplished
  Significance Publication/ Treatment
10. Work Screening of serum folic acid and serum Vit B12 in Anemic children
  Status of the project Project in pipeline
  Significance Publication/ Treatment
11. Work Role of total oxidant and total antioxidant level in the Development of vitiligo
  Status of the project Project in pipeline
  Significance Publication/ Treatment
12. Work Screening for the presence of NDM-I carbapamanase Gene in the bacterial isolates of SRMS, IMS by PCR Technique
  Status of the project Project in pipeline
  Significance Publication/ Treatment

1. Work i) Paper-Chromatography
ii) Thin layer – Chromatography
iii) Gel permeation – Chromatography
iv) Agarose gel – Electrophoresisv
v) SDS – PAGE
vi) Serum protein – electrophoresis
vii) DNA extraction from peripheral
viii) PCR
ix) Western blotting
x) DNA extraction from culture
xi) Plasmid DNA extraction from E. coli
xii) Quantification and purity of Nucleic acids
xiii) Spectrophotometry
  Significance Practicals  
  Work done for Faculty and PG students  

1. Work Mentoring will be provided for their research thesis
  Status of the project Research thesis
  Work done for/done by PG students (Dr. Z. Ali, Dr. Jaspreet Kaur & Nausheen Zakir)